Fig 2: The morphological images of Porella grandiloba. (A) Dorsal view of a specimen plant and (B) median cells of a leaf were captured using stereomicroscope (Leica S9i) and light microscope (Leica DM750), respectively.

Fig 3: (a) Representative images of embryos obtained from a parn heterozygous cross at day 3. (b–d) shows the electropherogram of wildtype, heterozygous (parn 5∆/+) and homozygous (parn 5∆/5∆) mutant embryos respectively. The region highlighted in ‘b’ shows 5 bases ‘CCTGA’ intact in wildtype. In ‘c’ the arrows represent the rearrangement of bases due to loss of 5 bases ‘CCTGA’ in one allele. The highlighted region in ‘d’ shows the stop codon (TAA) that arises due to loss of 5 bases ‘CCTGA’ in both the allele. (e), (f), (g) corresponds to embryos at day 3 for wildtype, heterozygous and homozygous mutant respectively with no morphological difference. All images were captured using Leica S9D, Camera MC190. Polypeak parser (yosttools.genetics.utah.edu/PolyPeakParser/) software was used to analyse the sequencing data.

Fig 4: Images under magnification (Leica S9D, Leica microsystems, Germany) of aedes refiki. A Right lateral view of a complete female. B Magnified right lateral view of the abdominal area, showing the scattered pale scales forming apical bands. C Enlarged dorsal view of the terga showing the scattered pale scales and the absence of the median longitudinal band

Fig 5: Representative images of embryos obtained from a parn heterozygous (parn 5∆/+) cross at 0hpf (a), 6hpf (b), 10hpf (c), 18hpf (d), 24hpf (e), 48hpf (f), 72hpf (g), 96hpf (h) and 120hpf (i) to show that all were phenotypical identical. All images were captured using Leica S9D, Camera MC190.